Journal: Bioactive Materials

Article Title: In situ self-assembled organoid for osteochondral tissue regeneration with dual functional units

doi: 10.1016/j.bioactmat.2023.04.002

Figure Lengend Snippet: In vitro chondrogenic and osteogenic pre-differentiation induction of MSC-seeded microcryogels. (A) Schematic of the chondrogenic induction process. (B) Toluidine blue and Alcian blue staining of control microcryogels and CH-Microcryogels after 7 d of chondrogenic induction. Relative gene expression of (C) COL2, (D) SOX9, and (E) ACAN. (F) Schematic of the osteogenic induction process. (G) Alizarin red staining of control microcryogels and OS-Microcryogels after 7 d of osteogenic induction. (H) Quantitative detection of ALP in MSCs after 7 d of osteogenic induction on control microcryogels and OS-Microcryogels. (I) Quantitative detection of ALP in MSCs on 2D culture plates. Relative gene expression of (J) RUNX2, (K) OCN, (L) COL1, and (M) ALP. (N) Atomic force microscopy (AFM) of microcryogels after 7 d of chondrogenic and osteogenic induction. F-actin and DAPI staining of MSCs cultured in microcryogels after 7 d of (O) chondrogenic induction and (P) osteogenic induction. (*p < 0.05, **p < 0.01, ***p < 0.005, n. s. represents no significant difference, n = 3).

Article Snippet: Human umbilical cord MSCs were acquired from EUBIO Technologies and cultured in MSC growth medium (BioWit Technologies), as previously described [ 22 ].

Techniques: In Vitro, Staining, Control, Gene Expression, Microscopy, Cell Culture

Journal: Journal of tissue science & engineering

Article Title: Human Bone Marrow Derived Mesenchymal Stem Cells Regulate Leukocyte-Endothelial Interactions and Activation of Transcription Factor NF-Kappa B

doi: 10.4172/2157-7552.S3-001

Figure Lengend Snippet: Supernatants collected from MSC culture (150,000cells/well) alone, and U937 culture (3×105/well and 7.5×105/well) alone did not contain any IL-10, as quantified by cytokine bead assay for flow cytometry (BD Biosciences, San Jose, CA). Both co-culture groups (in ratios 1:2 and 1:5, MSCs:U937s) significantly increased IL-10 production in response to stimulation with LPS (5ng/mL).

Article Snippet: MSCs were cultured in MSC growth media (MSCGM, Lonza); PECs were maintained in EGM-2MV media from Lonza.

Techniques: Flow Cytometry, Co-Culture Assay

Journal: Journal of tissue science & engineering

Article Title: Human Bone Marrow Derived Mesenchymal Stem Cells Regulate Leukocyte-Endothelial Interactions and Activation of Transcription Factor NF-Kappa B

doi: 10.4172/2157-7552.S3-001

Figure Lengend Snippet: A. Schematic of adhesion molecule assay: U937 leukocytes (500,000cells/well) in 6 well plates were cultured with or without MSCs (ratio of 1:5) for 24 hours. The cells were stimulated with PMA (200ng/mL) for 20 minutes. The cells were then labeled with fluorophore conjugated antibodies to CD62L, CD29, CD11b, and CD18. B. Image U937: Representative image of U937 leukocytes alone in culture. C. Image U937 and MSC co-culture: Representative image of U937 leukocytes in co-culture with MSCs. D. Mean Flourescent Intensity changes in all markers (n=4): There were no differences in adhesion molecule expression in U937 leukocytes after co-culture with MSCs and stimulation with PMA. Adhesion molecule expression was quantified by mean fluorescence intensity for four individual culture wells.

Article Snippet: MSCs were cultured in MSC growth media (MSCGM, Lonza); PECs were maintained in EGM-2MV media from Lonza.

Techniques: Cell Culture, Labeling, Co-Culture Assay, Expressing, Fluorescence